MODIFICATION OF THE TRANSCRIPTION FACTOR FOR GLYCINE MAX TO INCREASE AFFINITY TO THE IN SILICO CAAT-MOTIF
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Abstract
Breeding new varieties of agricultural crops that are resistant to aggressive environmental conditions without reducing productivity is an important and urgent task. Modern methods of genetics and breeding make it possible to cross varieties in order to develop useful qualities and consolidate them in the population, based on genome mapping data with already annotated traits and genes. The literature offers various markers that are associated with the desired properties, such as microsatellite DNA regions (SSRs) and single nucleotide polymorphisms (SNPs), or are its direct initiator. Quantitative trait loci (QTLs) and transcription factors (TFs) are considered as initiators. If QTLs are required to increase the level of expression, then TFs are one of the key mechanisms for solving this problem. The transcription factor NFYC4 annotated in Arabidopsis thaliana plays an important role in the regulation of the plant's immune response to biotic stress and to the increase in the total protein level. For Glycine max, a homologous analogue has been presented that is responsible for the same functions. At this stage, this protein has an index of I1KC24. An increase in the expression of target genes for this protein is an important task. Protein engineering makes it possible to supplement the process of creating new stress-resistant varieties, due to additional modifications of the identified genes that are responsible for the expression of the necessary properties. In this work, one of the strategic approaches is proposed, which consists in increasing the affinity of a transcription factor for its regulatory region in DNA in silico in order to increase expression. An increase in affinity for the CAAT motif was achieved due to the introduction of multiple amino acid substitutions at the binding site for aspartic acid.
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